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300–500 mL of water from the filter chamber into a sterile 1 L bottle. Use the same or a new bottle to scoop sand from the chamber, and pour the sand into the bottle making sure that it is completely covered by water. b. Cartridge filters: Cut a portion of the filter to fit inside a 1 L bottle and add enough water from the filter chamber to

Jul 01, 2021 · Preparation of Alkaline Peptone Water. To make about 50 bottles (i.e. 500 ml), you will need 5 g of peptone and 5 g of Sodium chloride. Dissolve the peptone and sodium chloride in the water. Adjust the reaction of the medium to pH 8.6 to 9.0 using 1 mol/L sodium hydroxide. Dispense the medium in 10 ml amounts in screw-cap bottles.

Jul 01, 2021 · 1 Mix 0.75 g nutrient agar and 1.3 g nutrient broth in 100 ml distilled water, and heat to 100°C to dissolve the ingredients (place the flask in a boiling water bath). 2 Dispense the medium in 5–7 ml amounts in screw-cap bottles. Sterilize by autoclaving (with caps loosened) at 121°C for 15 minutes. When cool, tighten the bottle caps.

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