Using a sterile pipette tip or toothpick, select a single colony from your LB agar plate . Drop the tip or toothpick into the liquid LB + antibiotic and swirl. Loosely cover the culture with sterile aluminum foil or a cap that is not air tight. Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator.
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Experiment Materials A roll or box of Mentos chewy mints (stick with the standard mint flavor for now) 2-liter bottle of diet soda (either diet or regular soda will work for this experiment, but diet soda is not sticky when you’re cleaning it up, and it will usually create a
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INDICATORS: Cresol red and thymol blue ***** Buffer, Phosphate, Sorensen's Stock solution a) = 9.08gm /1000ml water of KH2-PO4 b) = 9.47gm /1000ml water of Na2-HPO4 Combine the two stock solutions Add 2 g of Charcoal, shake and let stand for 10 min. Filter.
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Water samples should be collected in a sterile 1-liter wide-mouth screw cap polypropylene plastic bottle. If the water source has been recently treated with chlorine, add 0.5 ml of 0.1N sodium thiosulfate to each 1 liter sample to neutralize the disinfectant.
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Bottles, jars, and jugs are commonly used in laboratories to contain, store, or transport dry or liquid substances. They are available in various sizes and materials. What Kinds of Lab Bottles Are There? Lab bottles are most often used for chemicals, solvents, and other liquids. Bottles typically have an opening that is smaller in diameter than
2021/7/1 · Semisolid Nutrient Agar. 1 Mix 0.75 g nutrient agar and 1.3 g nutrient broth in 100 ml distilled water, and heat to 100°C to dissolve the ingredients (place the flask in a boiling water bath). 2 Dispense the medium in 5–7 ml amounts in screw-cap bottles. Sterilize by autoclaving (with caps loosened) at 121°C for 15 minutes.
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