Pipette aseptically 10 ml of the thoroughly mixed sample into a sterile glass bottle with a ground glass stopper and add 90 ml of diluent to give a 1:10 dilution v/v. Alternatively, weigh, with aseptic precautions, 10 g of the thoroughly mixed sample into the bottle and add 90 ml of diluent to give a 1:10 dilution w/v, which, for all practical purposes, is equivalent to a 1:10 dilution w/w.
