Methods. In 500mL media bottle, add 10g of agar powder. Add 395mL H2O. To make cubes, melt the agar, Add 50mL .01M NaOH, and 5 mL Bromocresol Purple. Stir for hours or overnight to fully dissolve the indicator. Autoclave and store. To make cubes, put the bottle in a warm bath to melt the agar. Once melted, mix gently and pour into square container.